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Key Documents

安全性情報

203391

Sigma-Aldrich

(-)-Blebbistatin

≥98% (HPLC), solid, ATPase inhibitor, Calbiochem®

別名:

(-)-Blebbistatin

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About This Item

実験式(ヒル表記法):
C18H16N2O2
CAS番号:
分子量:
292.33
MDL番号:
UNSPSCコード:
12352200
NACRES:
NA.77

product name

(-)-Blebbistatin, The active enantiomer of (±)-Blebbistatin that accounts for the inhibitory activity towards ATPase and myosin II-dependent cellular processes.

品質水準

アッセイ

≥98% (HPLC)

形状

solid

有効性

2 μM IC50

メーカー/製品名

Calbiochem®

保管条件

OK to freeze
protect from light

yellow

溶解性

methanol: 1.5 mg/mL
100% DMSO: 100 mg/mL
90% DMSO: 75 mg/mL

輸送温度

wet ice

保管温度

−20°C

InChI

1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3/t18-/m1/s1

InChI Key

LZAXPYOBKSJSEX-GOSISDBHSA-N

詳細

The active enantiomer of (±)-Blebbistatin (Cat. No. 203390) that accounts for the inhibitory activity towards ATPase (IC50 = ~2 µM) and myosin II-dependent cellular processes. Also reported to potently inhibit the actomyosin ATPase activities of expressed smooth muscle myosin IIA (SMA) and smooth muscle myosin IIB (SMB) smooth muscle myosin II heavy-chain isoforms (IC50 ~3 µM).
The active enantiomer of (±)-Blebbistatin (Cat. No. 203390) that accounts for the inhibitory activity toward ATPase (IC50 ~2 µM) and myosin II-dependent cellular processes. Also reported to potently inhibit the actomyosin ATPase activities of expressed smooth muscle myosin IIA (SMA) and smooth muscle myosin IIB (SMB) smooth muscle myosin II heavy-chain isoforms (IC50 ~3 µM).

生物化学的/生理学的作用

Cell permeable: no
Product does not compete with ATP.
Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month in 90% DMSO or methanol (-20°C). Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

その他情報

Eddinger, T.J. et al. 2007. J. Pharmacol. Exp. Ther.320, 865.
Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類コード

11 - Combustible Solids

WGK

WGK 3


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

203392-VMG:
203391-0MG:
203391-1MG:
203391-VMG:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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文書ライブラリにアクセスする

Andrew D Doyle et al.
Developmental cell, 56(6), 826-841 (2021-03-12)
We describe a cellular contractile mechanism employed by fibroblasts and mesenchymal cancer cells to migrate in 3D collagen gels. During 3D spreading, fibroblasts strongly deform the matrix. They protrude, polarize, and initiate migration in the direction of highest extracellular matrix
Dennis J Yuan et al.
Biomaterials, 273, 120797-120797 (2021-04-21)
T cell activation is sensitive to the mechanical properties of an activating substrate. However, there are also contrasting results on how substrate stiffness affects T cell activation, including differences between T cells of mouse and human origin. Towards reconciling these
Takeshi Shimizu et al.
Journal of neurochemistry, 150(2), 158-172 (2018-12-28)
Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimulation can be transduced into intracellular biochemical signals through mechanosensors, such as integrin, p130Cas, talin and vinculin. However
Adam W Watson et al.
Cell reports, 35(13), 109293-109293 (2021-07-01)
While the immediate and transitory response of breast cancer cells to pathological stiffness in their native microenvironment has been well explored, it remains unclear how stiffness-induced phenotypes are maintained over time after cancer cell dissemination in vivo. Here, we show that
Takeshi Shimizu et al.
Glia, 65(2), 360-374 (2016-11-04)
Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimuli can be transduced into intracellular biochemical signals through mechanosensors and intracellular mechanotransducers, such as YAP. However, the

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