C3260
Citrate Synthase from porcine heart
ammonium sulfate suspension, ≥100 units/mg protein
Sinonimo/i:
Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Origine biologica
Porcine heart
Livello qualitativo
Stato
ammonium sulfate suspension
Attività specifica
≥100 units/mg protein
PM
98 kDa ( 49 kDa monomer)
Solubilità
H2O: soluble 1.0 mg/mL, clear
Attività estranea
isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%
Temperatura di conservazione
2-8°C
Applicazioni
Citrate Synthase from porcine heart has been used:
- to inject newt egg for determining its importance in egg activation at fertilization
- in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
- to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs
Azioni biochim/fisiol
Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Definizione di unità
One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.
Stato fisico
Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.
Nota sulla preparazione
Dissolves in water to form a clear solution at 1 mg/mL concentration.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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