T6258

Triosephosphate Isomerase from rabbit muscle

Type X, lyophilized powder, ≥3,500 units/mg protein

• Sinonimo/i: D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
• Numero CAS: 9023-78-3
• Classificazione EC (Enzyme Commission): 5.3.1.1 (BRENDA, IUBMB)
• Numero CE: 232-774-2
• Numero MDL: MFCD00082080
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Tipo: Type X
• Forma fisica: lyophilized powder
• Attività specifica: ≥3,500 units/mg protein
• PM: calculated mol wt 53.2 kDa
• Composizione: Protein, 60-90% biuret
• Attività estranea: pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state.

Applicazioni

Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS).

Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure.

Azioni biochim/fisiol

Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease.

Confezionamento

Package size based on protein content

Definizione di unità

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Stato fisico

Sulfate-free, contains EDTA and borate buffer salts

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)