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Key Documents

CS0720

Sigma-Aldrich

Citrate Synthase Assay Kit

1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

Sinonimo/i:

Citrate Synthase Activity Test Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12161503
NACRES:
NA.28

Livello qualitativo

impiego

 kit sufficient for 100 reactions (using a 1 ml cuvette)
 kit sufficient for 480 reactions (using 96 multiwell plates)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CS(1431)

Descrizione generale

The Citrate Synthase Assay Kit contains all the required reagents (including a positive control enzyme) for a fast and simple measurement of citrate synthase activity in a whole cell extract or in isolated mitochondria. In addition, the kit enables testing of the intactness of the mitochondrial inner membrane.

Azioni biochim/fisiol

Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate. This enzyme is an exclusive marker of the mitochondrial matrix.

Risultati analitici

The activity of the enzyme is measured by following the color of TNB, which is generated from DTNB present in the reaction of citrate synthesis, and caused by the deacetylation of Acetyl-CoA. The overall reaction product, TNB, absorbs at 412 nm.

Solo come componenti del kit

N° Catalogo
Descrizione

  • Assay buffer for citrate synthase 5x 25 mL

  • Bicine buffer 10 mL

  • CelLytic M Cell Lysis Reagent 10 mL

  • DTNB [5,5'-Dithiobis-(2-nitrobenzoic acid)] 4 mg

  • Acetyl coenzyme A 25 mg

  • Oxaloacetic acid 1 g

  • Citrate synthase (positive control) .1 mL

Pittogrammi

Health hazardExclamation mark

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

10 - Combustible liquids


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