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Supelco

TOYOPEARL® DEAE-650M Bulk Media

phase DEAE (diethylaminoethyl), bottle of 250 mL, 65 μm particle size

Sinônimo(s):

TOYOPEARL® DEAE-650M Bulk Media

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About This Item

Código UNSPSC:
47101511

descrição

DEAE-650M

forma

slurry

Características

counter ion Cl-

embalagem

bottle of 250 mL

Parâmetros

3 bar max. pressure

técnica(s)

HPLC: suitable

matriz

methacrylate

Grupo ativo da matriz

DEAE (diethylaminoethyl) phase

tamanho de partícula

65 μm

pH operacional

2-10

capacidade

0.10 meq/mL±0.02 meq/mL ion exchange capacity
 30 g/L±5 adsorption capacity (BSA)
 30 mg/mL±5 mg/mL binding capacity (bovine serum albumin)

compatibilidade

mode of use weak anion exchange chromatography

técnica de separação

anion exchange

Descrição geral

Toyopearl DEAE-650 resin is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSKgel DEAE-5PW resin should be used. Selectivity remains the same.

Aplicação

TOYOPEARL® DEAE-650M is used in separation media and ion exchange resins. TOYOPEARL® DEAE-650M has been used in haemophilia studies.

Especificações

Clean in place with 0.5 M NaOH or 0.1 M HCl.

forma física

Shipped in 20% (v/v) ethanol.

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Informações legais

Toyopearl is a registered trademark of Tosoh Corporation

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Declarações de precaução

P210 - P233 - P240 - P241 - P242 - P243

Classificações de perigo

Flam. Liq. 3

Classe de risco de água (WGK)

WGK 1

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
107H0708

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Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

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Sigma-Aldrich

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Masahiko Takanashi et al.
Journal of bioscience and bioengineering, 97(1), 78-81 (2005-10-20)
D(-)-3-hydroxybutyrate dehydrogenase (BDH; EC 1.1.1.30) from a poly(D(-)-3-hydroxybutyrate) (PHB) degrading bacterium, Acidovorax sp. SA1, was purified using Toyopearl DEAE-650M, red-Sepharose CL-4B, and Q Sepharose FF. The molecular mass of the enzyme was estimated as 27 kDa by SDS-PAGE and 110
P R Foster et al.
Vox sanguinis, 86(2), 92-99 (2004-03-17)
The risk of haemophiliacs contracting variant Creutzfeldt-Jakob disease (vCJD) via treatment with factor VIII concentrates is not known. Therefore, in order to determine the extent to which the vCJD agent might be removed during the preparation of factor VIII concentrate
Christine Machold et al.
Journal of biotechnology, 117(1), 83-97 (2005-04-16)
Two different approaches of matrix assisted refolding have been evaluated and compared to conventional refolding by dilution. Bovine alpha-lactalbumin was used for the studies as model protein. It was adsorbed under denaturing conditions on an ion exchange matrix and refolding
Günter Iberer et al.
Journal of chromatography. A, 972(1), 115-129 (2002-10-25)
Preparative continuous annular chromatography, a method to separate proteins in a truly continuous manner, was investigated in an industrial environment. Plasma-derived clotting factor IX concentrate was used as model protein. Separation of vitronectin, a common impurity in commercial available factor

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