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GE17-0575-01

Chelating Sepharose Fast Flow

Cytiva 17-0575-01, pack of 50 mL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

iminodiacetic acid

embalagem

pack of 50 mL

fabricante/nome comercial

Cytiva 17-0575-01

matriz

6% cross-linked agarose

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning in place

2-14

working range

3-13

adequação

suitable for bioprocess medium

Descrição geral

Chelating Sepharose Fast Flow is a BioProcess IMAC medium, designed for purifying native and recombinant proteins and peptides that have an affinity for metal ions.

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

Chelating Sepharose Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.

Chelating Sepharose Fast Flow is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, Chelating Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

Características e benefícios

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of native and recombinant proteins
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • BioProcess medium supported for industrial applications and well-established in approved processes

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

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Artigos

This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

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