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Documentos Principais

A25120

Sigma-Aldrich

DEAE–Sephadex® A-25 chloride form

Sinônimo(s):

DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®

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About This Item

Número CAS:
12609-80-2
Número MDL:
MFCD00132778
Código UNSPSC:
47101511
NACRES:
NA.56

Formulário

powder

técnica(s)

activity assay: suitable

Matriz

cross-linked dextran

Grupo ativo da matriz

functional group

tamanho da esfera

40-125 μm (dry)

tamanho de poro

~30,000 Da exclusion limit

pH operacional

2-9

capacidade

3-4 meq/g ion exchange capacity

compatibilidade

mode of use mixed weak and strong anion exchange chromatography

Descrição geral

The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.

A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02

Aplicação

DEAE-Sephadex® A-25 has been used:
  • to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
  • in the estimation of GLS content in Chinese kale sprouts
  • production and purification of endozepine from astrocytes
  • to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients

Características e benefícios

  • Weak anion exchanger
  • Suitable for batch techniques
  • High binding capacities
  • Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

Informações legais

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

substituído por

Nº do produto
Descrição
Preços

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000274266
SLCN0054
SLCN0054
0000274266
SLCM3767

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Sigma-Aldrich

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vibrant-m

Y0000744

Dextranomer

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Sigma-Aldrich

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Sigma-Aldrich

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In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle.
P H Jones
Experimental cell research, 139(2), 401-404 (1982-06-01)
H Wang et al.
Neuroscience, 46(4), 793-806 (1992-01-01)
We previously isolated two glutamate-binding proteins from rat brain synaptic membranes of 71,000 and 63,000 mol. wt [Chen et al. (1988) J. biol. Chem. 263, 417-426]. In the present study, the 71,000 and 63,000 mol. wt glutamate-binding proteins were purified
W Sun et al.
The Journal of biological chemistry, 270(38), 22271-22276 (1995-09-22)
Epitopes for monoclonal antibodies directed against the purified adult rat skeletal muscle sodium channel (rSkM1) were localized using channel proteolysis and fusion proteins. The interactions between these and other monoclonal antibodies with site-specific polyclonal antibodies were used to investigate the
Isolation and study of insulin activated nitric oxide synthase inhibitory protein in acute myocardial infarction subjects.
Ray, U., et al.
Journal of Thrombosis and Haemostasis, 33(3), 218-229 (2012)
Human Lymphocyte Agglutinins in <I>Tursiops Truncatus</I> (Bottlenose Dolphin).
Hohn, D., et al.
Immunological Investigations, 12(5), 481-490 (1983)
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