T3287

Monoclonal Anti-Talin antibody produced in mouse

clone 8d4, ascites fluid

• Sinônimo(s): Monoclonal Anti-Talin
• Número MDL: MFCD00164744
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: ascites fluid
• tipo de produto de anticorpo: primary antibodies
• clone: 8d4, monoclonal
• peso molecular: antigen 225-235 kDa
• contém: 15 mM sodium azide
• reatividade de espécies: chicken, frog, rat, human, mouse
• técnica(s): immunoprecipitation (IP): suitable; indirect immunofluorescence: 1:500 using cultured Rat2 cells; western blot: 1:100 using chicken gizzard extract
• Isotipo: IgG1
• nº de adesão UniProt: Q9Y490
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TLN1(7094) , TLN2(83660) ; mouse ... Tln1(21894) , Tln2(70549) ; rat ... Tln1(313494)

Descrição

Descrição geral

Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the 8d4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Talin is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin.

Talin 1 (TLN1) gene mapped to human chromosome 9p13.3, codes for a 270kDa cytoskeletal protein containing 2541 amino acids. Talin-1 is widely expressed in the kidney, liver, spleen, stomach, lung and vascular smooth muscle. The TLN1 antiparallel dimeric structure is characterized with a globular head containing four-point-one, ezrin, radixin, moesin (FERM) domain and rod domain involved in dimer formation.

Especificidade

Reacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular weight talin in human platelets. The antibody labels focal adhesions, membrane ruffles, and ventral streaks in methanol/acetone fixed cultured fibroblasts.

Imunogênio

purified chicken gizzard talin.

Aplicação

Monoclonal Anti-Talin may be used for the localization of talin using various immunochemical assays such as immunoblot, immunocytochemistry, immunofluorescence, enzyme linked immunosorbent assay (ELISA) and for microinjection and transfection studies. The antibody recognizes native talin and can be used for immunoprecipitation.

Ações bioquímicas/fisiológicas

Talin 1 (TLN1) is a cytoskeletal protein, which plays a vital role in cancer cell adhesion, migration and invasion. It is also involved in integrin activation and it facilitates the adhesion of integrin to actin cytoskeleton. TLN1 might act as a potential prognostic biomarker in nasopharyngeal carcinoma (NPC). Overexpression of TLN1 is observed in endometrioid carcinoma, oral squamous cell carcinoma (OSCC), prostate cancer and hepatocellular carcinoma. Serum TLN1 functions as a potential biomarker of hepatocellular carcinoma (HCC). Talin 1 (TLN1) is highly expressed in glioblastoma multiforme (GBM). The Inhibition of TLN1 activity helps in diminishing malignant features of GBM cells. It also plays an important role in regulation of, Bevacizumab (anti-angiogenic agent)-resistance.

Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable