V9264

Anti-Vinculin Antibody

mouse monoclonal, hVIN-1

• Sinônimo(s): Monoclonal Anti-Vinculin antibody produced in mouse
• Número MDL: MFCD00162905
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• Nome do produto: Anti-vinculina monoclonal, clone hVIN-1, purified from hybridoma cell culture
• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: hVIN-1, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen 116 kDa
• reatividade de espécies: frog, chicken, mouse, canine, human, bovine, rat, turkey
• embalagem: antibody small pack of 25 μL
• concentração: ~1.0 mg/mL
• técnica(s): immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.05-0.1 μg/mL using HS-68 total cell extract
• Isotipo: IgG1
• nº de adesão UniProt: P18206
• aplicação(ões): research pathology
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

Descrição

Descrição geral

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

Especificidade

Marca especificamente a vinculina nos contatos célula-célula e célula-substrato. Reage fortemente com a vinculina humana. Mostra a reatividade cruzada com a metavinculina do músculo liso.

Imunogênio

vinculina humana purificada de útero.

Aplicação

Anti-Vinculin antibody, Mouse monoclonal has been used in

• immunoblotting
• immunofluorescence staining
• immunocytochemistry
• immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

Ações bioquímicas/fisiológicas

Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)