A8481
Anti-γ-Actin antibody, Mouse monoclonal
clone 2-2.1.14.17, purified from hybridoma cell culture
Sinônimo(s):
Anti-ACTG1
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
purified from hybridoma cell culture
tipo de produto de anticorpo
primary antibodies
clone
2-2.1.14.17, monoclonal
Formulário
buffered aqueous solution
peso molecular
antigen ~43 kDa
reatividade de espécies
mouse, chicken, canine, bovine, human, hamster
embalagem
antibody small pack of 25 μL
concentração
~2 mg/mL
técnica(s)
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells
Isotipo
IgG1
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
bovine ... ACTG1(404122)
chicken ... ACTG1(415296)
human ... ACTG1(71)
mouse ... Actg1(11465)
Descrição geral
γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.
Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH2-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms.
Especificidade
Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin.
Aplicação
Anti-γ-Actin antibody, Mouse monoclonal has been used in :
- immunofluorescence
- enzyme linked immunosorbent assay (ELISA)
- western blotting
- immunohistochemistry
Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays.
Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading.
Ações bioquímicas/fisiológicas
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.
forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Outras notas
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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