SAB4200283

Anti-Tensin 1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Sinônimo(s): Anti-TNS1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• peso molecular: antigen 225 kDa (rat); antigen ~150 kDa (human)
• reatividade de espécies: rat, human, chicken
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• concentração: ~1.0 mg/mL
• técnica(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat heart.; indirect immunofluorescence: 3-6 μg/mL using chicken embryo fibroblast cells.; western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human tensin 1
• nº de adesão UniProt: Q9HBL0
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TNS1(7145) ; mouse ... Tns1(21961) ; rat ... Tns1(301509)

Descrição

Descrição geral

Tensin 1 (TNS1) is encoded by the gene mapped to human chromosome 2q35. The encoded protein is localized to focal adhesions and is mainly expressed in renal tubular epithelial cells (TECs).

Imunogênio

synthetic peptide corresponding to a sequence located in the internal region of human tensin1, conjugated to KLH. The corresponding sequence is identical in rat and mouse tensin1.

Aplicação

Anti-Tensin 1 antibody may be used in immunofluorescence and immunohistochemistry.

Anti-Tensin 1 antibody produced in rabbit has been used in immunostaining and immunoblotting.

Ações bioquímicas/fisiológicas

Tensin 1 (TNS1) contributes to transforming growth factor (TGF)-β-induced myofibroblast differentiation and extracellular fibronectin and collagen matrix formation. Disruption in the gene and associated signaling might hinder tissue fibrosis. The encoded protein anchors to stress fibers by interacting with cytoplasmic tails of integrins and increases metastatic abilities of cancer cells. Tensin plays a vital role in activation of the c-Jun N-terminal kinases (JNK) and p38 pathways.

Tensin 1 associates with actin, integrin, FAK, p130Cas, DLC-1 and protein phosphatase PP1 which affects cell polarization, migration and invasion. Tensin 1 undergoes extensive Ser/Thr and Tyr phosphorylation induced by extracellular matrix (ECM), various growth factors and oncogenes. In addition, tensin 1 is cleaved by calpain II, a focal adhesion protease, suggesting that tensin 1 plays important roles in organizing the actin cytoskeleton and mediating signal transduction.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable