SRE0041

Catalase from bovine liver

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 2,000-5,000 units/mg protein

• Sinônimo(s): Catalase from bovine liver, H₂O₂:H₂O₂ oxidoreductase
• Número CAS: 9001-05-2
• Número da licença da enzima: 1.11.1.6 (BRENDA, IUBMB)
• Número CE: 232-577-1
• Número MDL: MFCD00081483
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• Nível de qualidade: 400
• forma: lyophilized powder
• atividade específica: 2,000-5,000 units/mg protein
• peso molecular: tetramer ~250 kDa
• ponto isoelétrico: 5.4
• aplicação(ões): diagnostic assay manufacturing
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• chave InChI: NUVBSKCKDOMJSU-UHFFFAOYSA-N

Descrição

Descrição geral

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Aplicação

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

• to prepare H₂O₂-O₂ based biocathode for applications in glucose biofuel cells
• to study the kinetic properties and storage stability of catalase immobilized on to florisil
• in glutathione-mediated superoxide generation in an aqueous solution

Ações bioquímicas/fisiológicas

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

Definição da unidade

One unit will decompose 1.0 micromole of hydrogen peroxide per minute at pH 7.0 and 25 deg C, while the hydrogen peroxide concentration falls from 10.3 to 9.2 millimolar.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable