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Key Documents

P1016

Sigma-Aldrich

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis

Grade I, buffered aqueous glycerol solution, 0.8-2.0 units/mg protein

Sinônimo(s):

L-Phenylalanine ammonia-lyase, Phenylalanine Deaminase from Rhodotorula glutinis

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About This Item

Número CAS:
Número da licença da enzima:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

fungus (Rhodotorula glutinis)

Nível de qualidade

tipo

Grade I

forma

buffered aqueous glycerol solution

atividade específica

0.8-2.0 units/mg protein

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

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Aplicação

Phenylalanine ammonia-lyase has been used in a study to assess the effect of heat treatment on lignification of postharvest bamboo shoots. It has also been used in a study to investigate the effect of light on gene expression and podophyllotoxin biosynthesis in Linum album cell culture.

Ações bioquímicas/fisiológicas

Phenylalanine Ammonia-Lyase from Rhodotorula glutinis can be cleaved and inactivated by the proteases chymotrypsin, subtilisin, and trypsin.

Definição da unidade

One unit will deaminate 1.0 μmole of L-phenylalanine to trans-cinnamate and NH3 per min at pH 8.5 at 30 °C.

forma física

Solution in 60% glycerol, 3 mM Tris-HCl, pH 7.5, containing up to 0.5 M (NH4)2SO4

Nota de análise

Protein determined by biuret

inibidor

Nº do produto
Descrição
Preços

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Zisheng Luo et al.
Food chemistry, 135(4), 2182-2187 (2012-09-18)
In order to evaluate the effects of heat treatment on the quality of postharvest bamboo shoots, the firmness, disease incidence, respiration and ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities, lignin and cellulose contents, and the activities of
Reid
Applied and environmental microbiology, 64(6), 2117-2125 (1998-06-03)
The fungus Trametes versicolor can delignify and brighten kraft pulps. To better understand the mechanism of this biological bleaching and the by-products formed, I traced the transformation of pulp lignin during treatment with the fungus. Hardwood and softwood kraft pulps
Ronald D Hatfield et al.
Planta, 229(6), 1253-1267 (2009-03-17)
Grasses are a predominant source of nutritional energy for livestock systems around the world. Grasses with high lignin content have lower energy conversion efficiencies for production of bioenergy either in the form of ethanol or to milk and meat through
Morteza Yousefzadi et al.
Plant physiology and biochemistry : PPB, 56, 41-46 (2012-05-15)
Podophyllotoxin (PTOX) is a naturally occurring phenolic compound isolated as an active anti-tumor agent. The stimulatory influence of light on the formation of phenolic compounds has been reported, but the molecular mechanism underlying the effect of light on the expression
H J Gilbert et al.
The Biochemical journal, 199(3), 715-723 (1981-12-01)
Phenylalanine ammonia-lyase (EC 4.3.1.5) of the yeast Rhodotorula glutinis was rapidly inactivated by duodenal juice. It was susceptible to chymotrypsin and subtilisin and to a lesser extent trypsin. Initial proteolysis of the enzyme by chymotrypsin and trypsin resulted in cleavage

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