C4963
Catalase−polyethylene glycol
lyophilized powder, ~40,000 units/mg protein
Sinônimo(s):
PEG-Catalase
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About This Item
Produtos recomendados
Formulário
lyophilized powder
Nível de qualidade
atividade específica
~40,000 units/mg protein
peso molecular
PEG 5,000
composição
Protein, ~50% E405
Extensão da rotulagem
~40 mol PEG per mol protein
ligação da matriz
secondary amine linkage.
temperatura de armazenamento
−20°C
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Aplicação
Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation.
Ações bioquímicas/fisiológicas
Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. It does not require any activators.
Embalagem
Package size based on protein content
Outras notas
Catalase from bovine liver coupled to methoxy-polyethylene glycol.
forma física
Contains PEG plus 5% citrate buffer salts
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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