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SAB4200740

Sigma-Aldrich

Anti-Neurofilament 160 antibody, Mouse monoclonal

clone NN18, purified from hybridoma cell culture

Sinônimo(s):

Anti-160 kDa neurofilament protein, Anti-NF-M, Anti-Neurofilament 3, Anti-Neurofilament triplet M protein

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

200

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

NN18, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~160 kDa

concentração

~1.0 mg/mL

técnica(s)

immunoblotting: 0.3-0.6 μg/mL using rat brain extract
immunofluorescence: 10-20 μg/mL using human bone marrow neuroblast SHSY5Y cell line
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain sections

Isotipo

IgG1

nº de adesão UniProt

P07197

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Anti-Neurofilament 160 antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the NN18 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with neurofilaments purified from pig spinal cord. Neurofilaments (NFs) belong to the intermediate filament (IF) family and are expressed mainly in cells or tissues of neuronal origin. Three major NFs subunits were discovered: light (NF-L, ~68 kDa), medium (NF-M, ~160 kDa) and heavy (NF-H, ~200 kDa), which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 160, also known as NF-M or Neurofilament triplet M protein.

Imunogênio

neurofilaments purified from pig spinal cord

Aplicação

Anti-Neurofilament 160 antibody has been used in immunoblotting, Immunohistochemistry, Immunofluorescence

Ações bioquímicas/fisiológicas

NFs can accumulate in large numbers within cell bodies and proximal axons of affected neurons in a variety of pathologies, including Charcot-Marie-Tooth disease (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, NFs accumulation were detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients. Elevated concentrations of Neurofilament 160 have been shown in cerebrospinal fluid (CSF) and serum samples from patients with brain injury.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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M M Black et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 8(9), 3296-3305 (1988-09-01)
The principal subunits of neurofilaments (NFs) of immature cultured sympathetic neurons have apparent Mr of 68,000 and 145,000; a 200,000 Mr subunit is also present, but at comparatively low levels. These subunits are referred to as the low (NFL), middle
G Shaw et al.
Brain research, 460(2), 227-234 (1988-09-20)
Previous immunocytochemical studies have shown immunological differences between neurofilaments in axons and those in dendrites and perikarya of many mature neuron types: it is now known that non-phosphorylated epitopes are normally seen in cell bodies and dendrites of mature neurons
M J Monteiro et al.
The Journal of cell biology, 108(2), 579-593 (1989-02-01)
We have used transient and stable DNA transfection to force synthesis of the mouse NF-L and NF-M genes in nonneuronal cultured animal cells. When the authentic NF-L gene (containing 1.7 kb of sequences 5' to the transcription initiation site) was
Eduardo Martínez-Morillo et al.
Clinical chemistry and laboratory medicine, 53(10), 1575-1584 (2015-02-27)
Brain injury is a medical emergency that needs to be diagnosed and treated promptly. Several proteins have been studied as biomarkers of this medical condition. The aims of this study were to: 1) evaluate the selectivity and precision of a
Elevated neurofilament levels in neurological diseases
Norgren N, et al.
Brain Research, 987(1), 25-31 (2003)
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