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Key Documents

AB1987

Sigma-Aldrich

Anti-Neurofilament M (145 kDa) Antibody, CT

serum, Chemicon®

Sinônimo(s):

Anti-NEF3

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

serum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

reatividade de espécies

reptile, chicken, rat, quail, pig, human, mouse, bovine, rabbit

fabricante/nome comercial

Chemicon®

técnica(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

bovine ... Nefm(281347)
chicken ... Nefm(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rabbit ... Nefm(100009343)
rat ... Nefm(24588)

Descrição geral

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.

Especificidade

Strong and specific staining for NF-M (145 kDa)

Imunogênio

Epitope: C-terminus
Recombinant fusion protein containing the C-terminal 168 amino acids of rat NF-M

Aplicação

Anti-Neurofilament M (145 kDa) Antibody, C-terminus detects level of Neurofilament M (145 kDa) & has been published & validated for use in IC, IH, IH(P) & WB.
Immunohistochemistry:
A 1:200 dilution of a previous lot was used in Immunohistochemistry.
Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum

Immunohistochemistry(paraffin):

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Qualidade

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..

Descrição-alvo

145 kDa

forma física

Neat rabbit antiserum containing no preservative.
Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Rat enteric neurons, rat spinal cord tissue Brain, Peripheral Nerve

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

T G Bush et al.
Neuron, 23(2), 297-308 (1999-07-10)
Reactive astrocytes adjacent to a forebrain stab injury were selectively ablated in adult mice expressing HSV-TK from the Gfap promoter by treatment with ganciclovir. Injured tissue that was depleted of GFAP-positive astrocytes exhibited (1) a prolonged 25-fold increase in infiltration
A hereditary spastic paraplegia mutation in kinesin-1A/KIF5A disrupts neurofilament transport.
Wang, L; Brown, A
Mol. Neurodegener. null
Birth of ophthalmic trigeminal neurons initiates early in the placodal ectoderm.
Kathryn L McCabe,John W Sechrist,Marianne Bronner-Fraser
The Journal of Comparative Neurology null
Interactions between peripherin and neurofilaments in cultured cells: disruption of peripherin assembly by the NF-M and NF-H subunits.
J M Beaulieu, J Robertson, J P Julien
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire null
Pool-specific regulation of motor neuron survival by neurotrophic support.
Lamballe, F; Genestine, M; Caruso, N; Arce, V; Richelme, S; Helmbacher, F; Maina, F
The Journal of Neuroscience null

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