N4142

Anti-Neurofilament 200 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinônimo(s): NF200 Antibody - Anti-Neurofilament 200 antibody produced in rabbit, Nf200 Antibody, AntiNeurofilament heavy chain
• Número MDL: MFCD02263444
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: IgG fraction of antiserum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• peso molecular: antigen 200 kDa
• reatividade de espécies: bovine, wide range
• embalagem: antibody small pack of 25 μL
• validação aprimorada: independent ( Antibodies); Learn more about Antibody Enhanced Validation
• técnica(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections; microarray: suitable; western blot: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... NEFH(4744) ; mouse ... Nefh(380684) ; rat ... Nefh(24587)

Descrição

Descrição geral

Neurofilament 200, also known as neurofilament heavy polypeptide (NEFH), is encoded by the gene mapped to human chromosome 22q12.2. The encoded protein has a molecular mass of 200kDa and belongs to the group of type IV intermediate filaments which are key constituents of the neuronal cytoskeleton. NEFH contains C-terminal motifs involved in the formation of sidearm projections which cross-link the neurofilaments.

Especificidade

Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide.

Imunogênio

Neurofilament 200 from bovine spinal cord.

Aplicação

Anti-neurofilament 200 antibody produced in rabbit has been used for immunolabeling.
Anti-neurofilament 200 antibody produced in rabbit has been used:

• for detection of neurofilaments using immunohistological staining
• in human astrocytoma cell line
• in post-mortem brain sections
• in human cremaster muscles
• for detection of neurofilaments by immunofluorescence staining in lumbar spinal cord sections of mice

Ações bioquímicas/fisiológicas

Neurofilament 200 plays a vital role in cytoarchitecture organization. Downregulated expression of the gene has been observed in schizophrenia patients. Loss of gene expression is associated with the development of prostate carcinoma. Mutation in the gene leads to sporadic amyotrophic lateral sclerosis (ALS).
Neurofilament 200 assembly during development is critical for normal functioning of neuronal cells. Mutations in gene encoding neurofilaments is associated with neurological disorder called the Charcot–Marie–Tooth.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

Store at -20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, solution may be frozen in working aliquots at –20°C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable