MAB5448
Anti-Neurofilament H (200 kDa) Antibody
CHEMICON®, rat monoclonal, TA51
Sinônimo(s):
NF-H
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
product name
Anti-Neurofilament NF-H Antibody, clone TA51, culture supernatant, clone TA51, Chemicon®
fonte biológica
rat
Nível de qualidade
forma do anticorpo
culture supernatant
tipo de produto de anticorpo
primary antibodies
clone
TA51, monoclonal
reatividade de espécies
human, rat, hamster, monkey
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... NEFH(4744)
rat ... Nefh(24587)
rhesus monkey ... Nefh(717705)
Especificidade
Neurofilament H (NF-H), carboxy terminal (phosphate dependent (+). Clone is specific for phosphorylated NF-H {Carden et al (1987) 7(11):3489}.
Imunogênio
Epitope: carboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH
Aplicação
Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Western blot: Identifies ~ 200kDa band in reduced samples. Use Tris based buffers for best results as epitope is phosphate dependent; Different regions of the CNS were dissected out immediately and homogenized in BUST buffer (8 M urea, 0.5% SDS, 2% 13-mercaptoethanol, and 50 mM Tris, pH 7.4) at a ratio of i ml/100 mg wet tissue. The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g
for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}
Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.
Optimal working dilutions must be determined by end user.
for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}
Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.
Optimal working dilutions must be determined by end user.
forma física
Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Armazenamento e estabilidade
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
recomendado
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Soundara Rajan Thangavelu et al.
Annals of neurosciences, 18(2), 48-55 (2011-04-01)
Amyotrophic Lateral Sclerosis, in which motor neurons degenerate, leading to paralysis, not only the affected motor neurons, but the surrounding non-neuronal cells also contribute significantly to the disease. However, the disease mechanism is not known. In this study we have
Natasha Sukhanov et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 41(7), 1393-1400 (2021-01-06)
Cell adhesion proteins of the Cadm (SynCAM/Necl) family regulate myelination and the organization of myelinated axons. In the peripheral nervous system (PNS), intercellular contact between Schwann cells and their underlying axons is believed to be mediated by binding of glial
Transgenic mice carrying a human mutant superoxide dismutase transgene develop neuronal cytoskeletal pathology resembling human amyotrophic lateral sclerosis lesions.
Tu, P H, et al.
Proceedings of the National Academy of Sciences of the USA, 93, 3155-3160 (1996)
Jean Defourny et al.
Nature communications, 4, 1438-1438 (2013-02-07)
Hearing requires an optimal afferent innervation of sensory hair cells by spiral ganglion neurons in the cochlea. Here we report that complementary expression of ephrin-A5 in hair cells and EphA4 receptor among spiral ganglion neuron populations controls the targeting of
Stimulation of adult oligodendrogenesis by myelin-specific T cells.
Hvilsted Nielsen, H; Toft-Hansen, H; Lambertsen, KL; Owens, T; Finsen, B
The American Journal of Pathology null
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica