ZMS1195

Anti-PVR/CD155 Antibody, clone SKII.4 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinônimo(s): CD155, NECL-5, Nectin-like protein 5, Poliovirus receptor
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: SKII.4, recombinant monoclonal
• descrição: recombinant, expressed in HEK 293 cells
• linha de produto: ZooMAb^® learn more
• forma: lyophilized
• peso molecular: calculated mol wt 45.3 kDa
• purificado por: using protein G
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μL
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnica(s): ELISA: suitable; affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable
• Isotipo: IgG1κ
• sequência de epítopo: Extracellular domain
• nº de adesão de ID de proteína: NP_006496.4
• nº de adesão UniProt: P15151
• categoria alternativa mais ecológica: Aligned
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone SKII.4 is ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Poliovirus receptor (PVR/CD155). It targets an epitope within the extracellular domain.

Imunogênio

SK-N-S1 human neuroblastoma cells.

Aplicação

Quality Control Testing

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected PVR/CD155 in HeLa cells.

Tested Applications

Flow Cytometry Analysis: 0.1 µg from a representative lot detected PVR/CD155 in one million Hela cells.

Affinity Binding Assay: A representative lot of this antibody bound His-tagged recombinant fragment corresponding to the extracellular domain of Human PVR/CD155 with a KD of 1.4 x 10-6 in an affinity binding assay.

Enzyme Immunoassay Analysis (ELISA): A serial of dilutions from a representative lot detected His-tagged recombinant fragment corresponding to the extracellular domain of Human PVR/CD155.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descrição-alvo

Poliovirus receptor (UniProt: P15151; also known as Nectin-like protein 5, NECL-5, CD155) is encoded by the PVR (also known as PVS) gene (Gene ID: 5817) in human. CD155 is a single-pass type I, N-glycosylated, membrane protein that mediates NK cell adhesion and triggers NK cell effector functions. It is ubiquitously expressed, and many human tumors are reported to significantly upregulate its expression. It binds two different NK cell receptors: CD96 and CD226 and these interactions accumulate at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and the target cell. This may trigger adhesion and secretion of lytic granules and IFN-γ and activate cytotoxicity of activated NK cells. CD155 serves as a receptor for poliovirus and may play a role in axonal transport of poliovirus by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with dynein light chain Tctex-type 1 (DYNLT1). It is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 21-343), a transmembrane domain (aa 344-367), and a cytoplasmic domain (aa 368-417). It contains two Ig-like C2 type domains (aa 145-237 and 233-328), and an Ig-like V-type domain that is shown to be necessary and sufficient for virus binding and uptake. CD155 also has one the immunoreceptor tyrosine-based inhibitor motif (ITIM; aa 396-401) that when phosphorylated by Src kinases binds to the SH2 domain of several SH2-containing phosphatases. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Sloan, KE., et al. (2004). BMC Cancer. 4; 73; Oda, T., et al. (2004). Biochem. Biophys. Res. Commun. 319(4);1253-1264; Mendelsohn, CL., et al. (1989). Cell. 56(5); 855-865).

forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstituição

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable