ZMS1199

Anti-PSGL-1/CD162 Antibody, clone KPL-1 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinônimo(s): P-selectin glycoprotein ligand 1, PSGL-1 Selectin P ligand
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: KPL-1, recombinant monoclonal
• descrição: recombinant, expressed in HEK 293 cells
• linha de produto: ZooMAb^® learn more
• forma: lyophilized
• purificado por: using protein G
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μL
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnica(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• sequência de epítopo: Extracellular domain
• nº de adesão de ID de proteína: NM_003006.3
• nº de adesão UniProt: Q14242
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone KPL-1 is a ZooMAb^® mouse monoclonal antibody that detects PSGL-1/CD162. It targets an epitope within the extracellular domain.

Imunogênio

PSGL-1 transfected murine 300.19 pre B-cells.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in HL-60 cell lysates.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PSGL-1/CD162 in HL-60 cell lysates.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PSGL-1/CD162 in lysate from Human peripheral blood mononuclear cells (PBMC)..

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PSGL-1/CD162 in Human tonsil tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected PSGL-1/CD162 in one million Human peripheral blood mononuclear cells (PBMC).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PSGL-1/CD162 in Jurkat cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

P-selectin glycoprotein ligand 1 (UniProt: Q14242; also known as PSGL-1 Selectin P ligand, CD162) is encoded by the SELPLG gene (Gene ID: 6404) in human. Leukocytes Express^™ L-selectin ligands that are critical for leukocyte-leukocyte interactions at sites of inflammation. PSGL-1 is a disulfide-linked, homodimeric protein. It serves as a predominant leukocyte L-selectin ligand that displays appropriate sialyl Lewis x (sLex)-like carbohydrate determinants for L-selectin recognition. Its expression is observed in neutrophils, monocytes, and most lymphocytes. It is constitutively expressed on all human peripheral-blood T cells and is also expressed on skin-homing T cells. It is a single-pass, mucin-like type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce a form that contains an extracellular domain (18-320), a transmembrane domain (321-341), and a cytoplasmic domain (342-412). The mature form is generated following the cleaved of the propeptide (aa 18-41) region. PSGL-1 is a SLe(x)-type proteoglycan, which through high Affinity^™, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Two isoforms of PSGL-1/CD162 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals.  (Ref.: Tu, L., et al. (1999). J. Immunol. 163(9); 5070-5078; Fuhlbrigge, RC., et al. (1997). Nature. 389(6654); 978-981; Duivestijn, AM., et al. (1988). Am. J. Pathol. 130(1); 147-155).

forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstituição

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

Affinity is a trademark of Mine Safety Appliances Co.

Express is a trademark of Merck KGaA, Darmstadt, Germany

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable