Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

Visualizar Toda a Documentação

M8787

Sigma-Aldrich

Magnesium chloride solution

PCR Reagent, 25 mM MgCI2 solution for PCR

Sinônimo(s):

PCR optimization

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Fórmula linear:
MgCl2
Número CAS:
7786-30-3
Peso molecular:
95.21
Número MDL:
MFCD00011106
Código UNSPSC:
12352302
ID de substância PubChem:
57654422
NACRES:
NA.52

grau

PCR Reagent

Nível de qualidade

200
300

Formulário

liquid

embalagem

vial of 1.5 mL

concentração

25 mM±1 mM

técnica(s)

PCR: suitable

cor

colorless

aplicação(ões)

agriculture
diagnostic assay manufacturing

atividade externa

DNase, RNase, none detected

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

chave InChI

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Aplicação

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression

Adequação

Suitable for optimization of polymerase chain reactions.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
SLCK6176
SLCH7551
SLCC7461
SLBZ9143
SLBQ4119V

Não está vendo a versão correta?

Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 7

1 of 7

Betaína 5 M, PCR Reagent

Sigma-Aldrich

B0300

Betaína

Taq DNA polimerase with 10× PCR reaction buffer without MgCl2

Sigma-Aldrich

D4545

Taq DNA polimerase

Glycogen azure ≥98% (HPLC), from rabbit liver, suitable for substrate for α-amylase

Sigma-Aldrich

G5510

Glycogen azure

Cloreto de magnésio anhydrous, ≥98%

Sigma-Aldrich

M8266

Cloreto de magnésio

Magnesium chloride anhydrous for synthesis

Sigma-Aldrich

8.14733

Magnesium chloride

Taq DNA polimerase with 10× PCR reaction buffer containing MgCl2

Sigma-Aldrich

D1806

Taq DNA polimerase

Cloreto de magnésio BioReagent, suitable for insect cell culture, ≥97.0%

Sigma-Aldrich

M4880

Cloreto de magnésio

Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Kadri Õunap et al.
PloS one, 10(7), e0133841-e0133841 (2015-07-28)
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the protein function in ribosome synthesis is independent of its enzymatic activity. In this work, we have
Manal A Farg et al.
Human molecular genetics, 23(13), 3579-3595 (2014-02-20)
Intronic expansion of a hexanucleotide GGGGCC repeat in the chromosome 9 open reading frame 72 (C9ORF72) gene is the major cause of familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. However, the cellular function of the C9ORF72 protein remains unknown.
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
Takeshi Tomita et al.
PloS one, 9(9), e108957-e108957 (2014-10-01)
Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins
Exibir todos os documentos relacionados

Artigos

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.).

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

Examination of Efficacy, Specificity and Efficiency of an shRNA Lentiviral Library

Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Protocolos

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica