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Documentos Principais

M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

Sinônimo(s):

MgCl2, dichloromagnesium, magnesium chloride

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About This Item

Fórmula linear:
MgCl2
Número CAS:
Peso molecular:
95.21
Número MDL:
Código UNSPSC:
12352302
ID de substância PubChem:
NACRES:
NA.52

grau

for molecular biology

Nível de qualidade

esterilidade

non-sterile; 0.2 μm filtered

Formulário

liquid

concentração

1.00 M±0.01 M

técnica(s)

DNA sequencing: suitable

Impurezas

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

solubilidade

water: soluble

densidade

1.080 g/cm3

traços de cátion

heavy metals (as Pb): ≤5 ppm

adequação

suitable for electrophoresis
suitable for molecular biology

aplicação(ões)

cell analysis

atividade externa

DNase, RNase, NICKase and protease, none detected

cadeia de caracteres SMILES

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

chave InChI

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Descrição geral

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Aplicação

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.
  • It has been used to protect the liver from oxidative damage.
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Características e benefícios

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

Componentes

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves


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Artigos

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

Protocolos

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