63069

Magnesium chloride solution

BioUltra, for molecular biology, ~1 M in H₂O

• Sinônimo(s): Magnesium Chloride hydrate, Magnesium chloride (MgCl₂)hexahydrate, MgCl₂
• Fórmula linear: MgCl₂
• Número CAS: 7786-30-3
• Peso molecular: 95.21
• Número MDL: MFCD00011106
• Código UNSPSC: 12352302
• ID de substância PubChem: 329759772
• NACRES: NA.26

Propriedades

• grau: for molecular biology
• Nível de qualidade: 200
• linha de produto: BioUltra
• Formulário: liquid
• concentração: ~1 M in H₂O
• técnica(s): HPLC: suitable; flow cytometry: suitable
• Impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.0002% total Nitrogen (N)
• pH: 5.0-7.5 (25 °C, 1 M in H₂O)
• solubilidade: water: soluble
• densidade: 1.08 g/mL at 20 °C
• traços de ânion: phosphate (PO₄^3-): ≤10 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• traços de cátion: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤10 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤50 mg/kg; Li: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤500 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• Absorção UV: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.03
• adequação: suitable for molecular biology
• cadeia de caracteres SMILES: Cl[Mg]Cl
• aplicação(ões): cell analysis; filtration; vaccine development
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• chave InChI: TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Descrição

Descrição geral

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg²⁺ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg²⁺ enhance the stability of primer-template duplexes and the efficiency of amplification.

Aplicação

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).

Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

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Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves