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M1302

Sigma-Aldrich

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

Sinônimo(s):

Moloney Murine Leukemia Virus Reverse Transcriptase

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About This Item

Número CAS:
9068-38-6
Número MDL:
MFCD00283754
Código UNSPSC:
12352202
NACRES:
NA.55

fonte biológica

Porcine intestinal mucosa

Nível de qualidade

200

recombinante

expressed in E. coli

forma

liquid

uso

sufficient for 200 reactions
 sufficient for 250 reactions

Características

dNTPs included: no
hotstart: no

concentração

200 units/μL

técnica(s)

RT-PCR: suitable

cor

colorless

entrada

purified RNA

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Descrição geral

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Aplicação

M-MLV Reverse Transcriptase has been used:
  • for the preparation of cDNA libraries or for first strand cDNA synthesis
  • for use in a 2-step RT-PCR assay
  • in quantitative realtime-polymerase chain reaction (RT-qPCR)
  • in reverse transcription

Características e benefícios

  • Thermostable reverse transcriptase active at 37 °C.
  • Can be used to generate first strand cDNA of up to 7 kb.

Embalagem

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Definição da unidade

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Nota de preparo

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Informações legais

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

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Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
<BIG><BIG>Guzik KA, et al.</BIG></BIG>
Acta Neurobiologiae Experimentalis, 71, 193-207 (2011)
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
Guzik-Kornacka A, et al.
Acta Neurobiologiae Experimentalis, 71(2), 193-207 (2011)
D S Howland et al.
Brain research. Molecular brain research, 11(3-4), 345-353 (1991-10-11)
The phosphoprotein synapsin I is expressed exclusively in neuronal cells. We are interested in elucidating the promoter sequences involved in cell type-specific expression of the synapsin I gene. The PC12 cell line expresses the 3.4 kb and 4.5 kb synapsin
Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana
Dikaios I, et al.
Photosynthesis Research, 1-16 (2019)
G F Gerard et al.
DNA (Mary Ann Liebert, Inc.), 5(4), 271-279 (1986-08-01)
We have cloned and expressed in Escherichia coli a section of the Moloney murine leukemia virus (Mo-MLV) pol gene which includes the entire coding region of mature reverse transcriptase (RT) plus 284 additional base pairs 3' to the coding region
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