11483188001
Roche
First Strand cDNA Synthesis Kit for RT-PCR (AMV)
sufficient for 30 reactions (including 5 control reactions), kit of 1 (10 components), suitable for RT-PCR, hotstart: no, dNTPs included
Sinônimo(s):
RT-PCR, cDNA sysnthesis
About This Item
Produtos recomendados
uso
sufficient for 30 reactions (including 5 control reactions)
Nível de qualidade
atividade específica
>50 units/μg protein
Características
dNTPs included
hotstart: no
embalagem
kit of 1 (10 components)
fabricante/nome comercial
Roche
Parâmetros
42 °C optimum reaction temp.
técnica(s)
RT-PCR: suitable
entrada
purified RNA
temperatura de armazenamento
−20°C
Descrição geral
AMV RT synthesizes the new cDNA strand at site(s) determined by the type of the primer used:
- at the 3′-end of the poly(A) mRNA when Oligo-p(dT)15is used as a primer,
- at nonspecific points along the mRNA template when using the random primer p(dN)6, or
- at a site determined by a sequence-specific primer.
Especificidade
Reverse Transcriptase AMV
Aplicação
- Detection of the presence or absence of RNA viruses or other RNA-containing microorganisms (in combination with PCR)
- Quantification of mRNA for monitoring differential expression of a specific mRNA
- First step in the "differential display of mRNA"
- Generation of cDNA libraries with large and full-length inserts
- Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for reverse transcription of RNA into complementary DNA.
Características e benefícios
- Transcribes total RNA, mRNA and viral RNA along with difficult-to-transcribe secondary RNA structures
- Obtain cDNA transcripts up to 12 kb
- Higher thermostability (up to 50°C) and specificity than M-MuLV Reverse Transcriptase
- Purification of the resulting cDNA before PCR reaction is not necessary
It can be used with either sequence-specific primers, poly(dT)15 primers, or random primers, p(dN)6
Embalagem
Qualidade
Definição da unidade
Outras notas
Somente componentes do kit
- Reverse Transcriptase AMV
- PCR Nucleotide Mix, pH 8.5 10 mM each
- Reaction Buffer
- MgCl2 Stock Solution 25 mM
- Gelatin 0.05% (w/v)
- Oligo-p(dT)15 , 0.02 A260 units/μl 0.8 μg/μl
- Random Primer p(dN)6 , 0.04 A260 units/μl 1.6 μg/μl
- RNase Inhibitor 50 U/μl
- Control Neo pa RNA (1.0 kb in length with additional 19-base 3′-poly(A) tail) 0.2 μg/μl
- Water, PCR Grade
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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Protocolos
First Strand cDNA Synthesis Kit for RT-PCR (AMV) Protocol
Conteúdo relacionado
RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.
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