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H4272

Sigma-Aldrich

Hialuronidase

Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

Sinônimo(s):

Hialuronato 4-glicanohidrolase, Hialuronoglicosaminidase

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About This Item

Número CAS:
37326-33-3
Número da licença da enzima:
3.2.1.35 (BRENDA, IUBMB)
Número CE:
253-464-3
Número MDL:
MFCD00131351
Código UNSPSC:
12352204
NACRES:
NA.75

esterilidade

aseptically processed

Nível de qualidade

200

tipo

Type IV-S

Formulário

powder

atividade específica

750-3000 units/mg solid

peso molecular

~55 kDa (four subunits of 14 kDa each)

composição

Protein, 80-105% biuret

técnica(s)

cell culture | embryo: suitable

temperatura de armazenamento

−20°C

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Aplicação

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass in the isolation of mouse embryos or from newly ovulated eggs, when preparing the eggs for in vitro culture.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.

Ações bioquímicas/fisiológicas

A hialuronidase degrada o hialuronano e demonstrou-se sua regulação inadequada durante a progressão de câncer.
Essas enzimas clivam aleatoriamente as ligações glicosídicas de β-N-acetilhexosamina-[1→4] em ácido hialurônico, condroitina e sulfatos de condroitina.

Definição da unidade

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Nota de preparo

Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
SLCJ3456
SLCJ0558
SLCD4050
SLCC6227
SLBX1080

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Liesl Jacobs et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(4), 1068-1077 (2020-02-27)
Checkpoint-inhibiting antibodies elicit impressive clinical responses, but still face several issues. The current study evaluated whether DNA-based delivery can broaden the application of checkpoint inhibitors, specifically by pursuing cost-efficient in vivo production, facilitating combination therapies, and exploring administration routes that lower
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Nature communications, 8(1), 2043-2043 (2017-12-13)
Bone formation in mammals requires continuous production of osteoblasts throughout life. A common molecular marker for all osteogenic mesenchymal progenitors has not been identified. Here, by lineage-tracing experiments in fetal or postnatal mice, we discover that Gli1
Wan Kin Au Yeung et al.
Cell reports, 27(1), 282-293 (2019-04-04)
Mammalian histone methyltransferase G9a (also called EHMT2) deposits H3K9me2 on chromatin and is essential for postimplantation development. However, its role in oogenesis and preimplantation development remains poorly understood. We show that H3K9me2-enriched chromatin domains in mouse oocytes are generally depleted
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
P Lorenzo Bozzelli et al.
Experimental neurology, 323, 113077-113077 (2019-11-05)
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