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F4125

Sigma-Aldrich

Ficin from fig tree latex

saline suspension, ≥1.0 units/mg protein (biuret)

Sinônimo(s):

Debricin, Ficain, higueroxyl delabarre

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About This Item

Número CAS:
9001-33-6
Número da licença da enzima:
3.4.22.3 (BRENDA, IUBMB)
Número MDL:
MFCD00081597
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

fig tree (latex)

Nível de qualidade

200

forma

saline suspension

atividade específica

≥1.0 units/mg protein (biuret)

peso molecular

23.8 kDa

temperatura de armazenamento

2-8°C

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Descrição geral

Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0

Aplicação

Ficin from fig tree latex has been used:
  • in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
  • for the digestion of eye tissue sections prior to immunostaining and immunofluorescence

Ações bioquímicas/fisiológicas

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.

Definição da unidade

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).

forma física

Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0

Nota de preparo

2× Crystallized

inibidor

Nº do produto
Descrição
Preços

substrato

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Lens-specific VEGF-A expression induces angioblast migration and proliferation and stimulates angiogenic remodeling
Ash JD and Overbeek PA
Developmental Biology, 223(2), 383-398 (2000)
Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex
Zare H, et al.
Phytochemistry, 87, 16-22 (2013)
Transgenic expression of leukemia inhibitory factor (LIF) blocks normal vascular development but not pathological neovascularization in the eye
Ash J, et al.
Molecular Vision, 11, 298-308 (2005)
Robertson D Davenport et al.
Transfusion, 44(6), 849-852 (2004-05-26)
Cefotetan can cause severe immune hemolytic anemia that may persist long after the drug is discontinued. To study the binding of cefotetan to RBCs, patients who received cefotetan were followed and tested for the presence of antibody to cefotetan. Patients
V B Reddy et al.
The British journal of dermatology, 163(3), 532-535 (2010-05-25)
Bromelain, ficin and papain are cysteine proteases from plants that produce itch upon injection into skin. Their mechanism of action has not been considered previously. To determine the mechanism by which these proteases function. The ability of these proteases to
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Protocolos

Enzymatic Assay of Ficin (EC 3.4.22.3)

This procedure may be used for all Ficin products.

Enzymatic Assay of Ficin (EC 3.4.22.3)

This procedure may be used for all Ficin products.

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