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Key Documents

F2043

Sigma-Aldrich

Anti-β-Tubulin−FITC antibody, Mouse monoclonal

clone TUB 2.1, purified from hybridoma cell culture

Sinônimo(s):

Monoclonal Anti-β-Tubulin

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

conjugado

FITC conjugate

forma do anticorpo

purified from hybridoma cell culture

tipo de produto de anticorpo

primary antibodies

clone

TUB 2.1, monoclonal

forma

buffered aqueous solution

peso molecular

antigen 55 kDa

reatividade de espécies

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

condição de armazenamento

protect from light

técnica(s)

direct immunofluorescence: 1:25 using cultured BHK cells

Isotipo

IgG1

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

β-Tubulin belongs to the subfamily of tubulins. It has two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain, which has the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). TUBB1 (tubulin beta class I) is located on human chromosome 6p21.33.(10)
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

Especificidade

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Imunogênio

tubulin from rat brain.

Aplicação

FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays.
Monoclonal Anti-β-Tubulin-FITC antibody has been used:
  • in the evaluation of the status of the meiotic spindle and the arrangement of chromosomes
  • in immunofluorescence studies
  • in immunofluorescence microscopy
  • in spindle and chromosome imagery(6)

Ações bioquímicas/fisiológicas

Mutation in β-Tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

Transient increase in the transcript levels of gamma-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls
Soga K, et al.
Journal of Plant Research, 121(5), 493-498 (2008)
Abraham L Kierszenbaum
Molecular reproduction and development, 62(1), 1-3 (2002-04-05)
Two microtubule-containing structures are assembled during spermiogenesis: a transient manchette and a stable axoneme. Both structures contain microtubules enriched in posttranslationally modified tubulins. Despite the existence of a spectrum of tubulin isotypes postulated by the multi-tubulin hypothesis, further extended by
Peloruside-and laulimalide-resistant human ovarian carcinoma cells have betaI-tubulin mutations and altered expression of betaII-and betaIII-tubulin isotypes
Kanakkanthara A, et al.
Molecular Cancer Therapeutics, 393(6681), molcanther-molcan1057 (2011)
Changes to the meiotic spindle and zona pellucida of mature mouse oocytes following different cryopreservation methods
Ko C S, et al.
Animal Reproduction Science, 105(3-4), 272-282 (2008)
J C Bruusgaard et al.
Journal of applied physiology (Bethesda, Md. : 1985), 100(6), 2024-2030 (2006-02-25)
We have recently published a new technique for visualizing nuclei in living muscle fibers of intact animals, based on microinjection of labeled DNA into single myofibers, excluding satellite cells (Bruusgaard JC, Liestol K, Ekmark M, Kollstad K, and Gundersen K.

Artigos

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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