F0635

Rat Fibronectin

from rat plasma, powder, suitable for cell culture

• Sinônimo(s): Fibronectin
• Número CE: 289-149-2
• Número MDL: MFCD00131062
• Código UNSPSC: 12352202
• eCl@ss: 42020141
• NACRES: NA.75

Propriedades

• Nome do produto: Fibronectina, powder, BioReagent, suitable for cell culture
• fonte biológica: rat plasma
• Nível de qualidade: 200
• esterilidade: sterile
• linha de produto: BioReagent
• Formulário: powder
• embalagem: pkg of 0.5 mg; pkg of 1 mg; pkg of 2 mg
• técnica(s): cell culture | mammalian: suitable
• cobertura de superfície: 1‑5 μg/cm²
• solubilidade: H₂O: 1 mg/mL at 37 °C, clear, colorless (some particles)
• nº de adesão UniProt: P04937
• Especificidade de ligação: Peptide Source: Collagen
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: rat ... Fn1(25661)

Descrição

Descrição geral

Fibronectin (FN)/FN1 (fibronectin 1) is a multifunctional glycoprotein, produced by hepatic stellate cells (HSCs). It is a constituent of extracellular matrix (ECM). FN1 is found in the cell membrane and cytoplasm. It is also synthesized and liberated by fibroblasts, endothelial cells, macrophages and hepatocytes in vivo.

Aplicação

Fibronectin rat plasma has been used in the induction of differentiation , adhesion to extracellular matrix, neural differentiation and primary neuronal ventral mesencephalon cultures.

Ações bioquímicas/fisiológicas

Fibronectin (FN)/FN1 (fibronectin 1) plays a major role in cell cycle progression, cell adhesion and proliferation and fibrotic progression. It is essential for for collagen matrix assembly. High expression of FN has a vital role during liver fibrosis. FN1 helps in the support of other matrix proteins.

Componentes

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

Atenção

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Nota de preparo

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, showing a single arc, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Nota de análise

A single arc is shown when reacted with anti-fibronectin by IEP (immunoelectrophoresis).

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)