Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos

10838039001

Roche

Fibronectina

from human plasma

Sinônimo(s):

Fibronectin

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(1)

About This Item

Número MDL:
MFCD00131062
Código UNSPSC:
12352207

fonte biológica

human plasma

Nível de qualidade

100

esterilidade

non-sterile; 0.2 μm filtered

Ensaio

95% (SDS-PAGE)

forma

lyophilized (clear, colorless solution after reconstitution)

peso molecular

440 kDa

embalagem

pkg of 5 mg

fabricante/nome comercial

Roche

condição de armazenamento

avoid repeated freeze/thaw cycles

técnica(s)

cell culture | mammalian: suitable

nº de adesão UniProt

P02751

temperatura de armazenamento

2-8°C

Informações sobre genes

human ... FN1(2335)

Procurando produtos similares? Visita Guia de comparação de produtos

Categorias relacionadas

Descrição geral

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Especificidade

Active on most mammalian cells

Aplicação

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.

Método de teste

Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)

forma física

Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.

Nota de preparo

Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstituição

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Os clientes também visualizaram

Slide 1 of 4

1 of 4

Fibronectina, plasma bovino powder, BioReagent, suitable for cell culture

Sigma-Aldrich

F4759

Fibronectina, plasma bovino

Fibronectina lyophilized powder, 45 kDa

Sigma-Aldrich

F0162

Fibronectina

Proteína purificada de fibronectina de plasma humano from human plasma, liquid, 1 mg/mL (100 MG pack size is lyophilized), purified protein, suitable for cell culture

Sigma-Aldrich

FC010

Proteína purificada de fibronectina de plasma humano

Superfibronectin from human plasma solution, BioReagent, suitable for cell culture

Sigma-Aldrich

S5171

Superfibronectin from human plasma

Daniella Penko et al.
Islets, 3(3), 73-79 (2011-04-12)
Pancreatic islet transplantation is limited by extensive apoptosis and suboptimal function of the implanted islets in the longer term. Endothelial progenitor cells (EPC) may be ideal for enhancing both the survival and function of transplanted islets. Here, we describe for
Daniella Penko et al.
Cell transplantation, 24(1), 37-48 (2013-09-28)
The success of pancreatic islet transplantation is limited by delayed engraftment and suboptimal function in the longer term. Endothelial progenitor cells (EPCs) represent a potential cellular therapy that may improve the engraftment of transplanted pancreatic islets. In addition, EPCs may
Isabel Fernández-Pisonero et al.
Cell reports, 38(11), 110522-110522 (2022-03-17)
A missense change in RRAS2 (Gln72 to Leu), analogous to the Gln61-to-Leu mutation of RAS oncoproteins, has been identified as a long-tail hotspot mutation in cancer and Noonan syndrome. However, the relevance of this mutation for in vivo tumorigenesis remains understudied.
Sayan Chakraborty et al.
Nature communications, 12(1), 6349-6349 (2021-11-05)
An orchestrated wound healing program drives skin repair via collective epidermal cell proliferation and migration. However, the molecular determinants of the tissue microenvironment supporting wound healing remain poorly understood. Herein we discover that proteoglycan Agrin is enriched within the early
Yi-Ting Yeh et al.
PLoS pathogens, 18(3), e1010309-e1010309 (2022-03-23)
The eggs of the parasitic blood fluke, Schistosoma, are the main drivers of the chronic pathologies associated with schistosomiasis, a disease of poverty afflicting approximately 220 million people worldwide. Eggs laid by Schistosoma mansoni in the bloodstream of the host
Exibir todos os documentos relacionados

Artigos

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolos

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica