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D2321

Sigma-Aldrich

DAF-FM DA solution

5 mM in DMSO, ≥97% (HPLC)

Sinônimo(s):

4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate

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About This Item

Fórmula empírica (Notação de Hill):
C25H18F2N2O7
Número CAS:
Peso molecular:
496.42
Número MDL:
Código UNSPSC:
12352200
NACRES:
NA.77

Nível de qualidade

Ensaio

≥97% (HPLC)

Formulário

DMSO solution

condição de armazenamento

desiccated
protect from light

cor

clear colorless

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

chave InChI

BEVHTVRRVVEMEF-UHFFFAOYSA-N

Ações bioquímicas/fisiológicas

DAF-FM DA is a cell permeable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Características e benefícios

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Outras notas

Light sensitive

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Aurora Parlati et al.
Frontiers in plant science, 8, 1299-1299 (2017-08-10)
The PII protein in plants has been associated to many different tissue specialized roles concerning the Nitrogen assimilation pathways. We report here the further characterization of
Diana Passaro et al.
Cancer cell, 32(3), 324-341 (2017-09-06)
The biological and clinical behaviors of hematological malignancies can be influenced by the active crosstalk with an altered bone marrow (BM) microenvironment. In the present study, we provide a detailed picture of the BM vasculature in acute myeloid leukemia using
Gen Li et al.
Frontiers in plant science, 11, 1215-1215 (2020-09-10)
Anthocyanin-derived fleshy fruit pigmentation has become an excellent system for studying the regulatory network underlying fruit ripening and quality. The transcriptional control of anthocyanin biosynthesis by MYB-bHLH-WDR complexes has been well established, but the intermediate signals through which the environmental
Caihua Wang et al.
Stem cell research & therapy, 10(1), 175-175 (2019-06-15)
Cancer stem cells (CSCs) play a critical role in tumor development and progression and are involved in cancer metastasis. The role of reactive oxygen species (ROS) in CSCs and cancer metastasis remains controversial. The aim of the present study was
Diego Piacentini et al.
Environment international, 132, 105094-105094 (2019-09-05)
Over the last years, various acellular assays have been used for the evaluation of the oxidative potential (OP) of particular matter (PM) to predict PM capacity to generate reactive oxygen (ROS) and nitrogen (RNS) species in biological systems. However, relationships

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