D1946

DAF-FM DA

≥98% (HPLC)

• Sinônimo(s): 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, Diaminofluorescein-FM diacetate
• Fórmula empírica (Notação de Hill): C₂₅H₁₈F₂N₂O₇
• Número CAS: 254109-22-3
• Peso molecular: 496.42
• Número MDL: MFCD16872050
• Código UNSPSC: 12352200
• ID de substância PubChem: 329798717
• NACRES: NA.77

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥98% (HPLC)
• forma: film
• condição de armazenamento: desiccated
• cor: colorless to yellow
• fluorescência: λ_ex 500 nm; λ_em 515 nm in DMSO
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• chave InChI: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Descrição

Aplicação

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Ações bioquímicas/fisiológicas

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Características e benefícios

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Atenção

Hygroscopic

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable