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D1821

Sigma-Aldrich

DAF-FM

≥98% (HPLC)

Sinônimo(s):

4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM

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About This Item

Fórmula empírica (Notação de Hill):
C21H14F2N2O5
Número CAS:
Peso molecular:
412.34
Número MDL:
Código UNSPSC:
12352204
ID de substância PubChem:
NACRES:
NA.77

Nível de qualidade

Ensaio

≥98% (HPLC)

Formulário

powder

condição de armazenamento

desiccated

cor

white to brown

solubilidade

DMSO: >1 mg/mL

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

chave InChI

DIJCILWNOLHJCG-UHFFFAOYSA-N

Aplicação

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Ações bioquímicas/fisiológicas

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Atenção

hygroscopic

Nota de preparo

Color variation within the range from white to brown does not change performance.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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