251765

DAR-4M AM

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• Sinônimo(s): DAR-4M AM, Diaminorhodamine-4M AM
• Fórmula empírica (Notação de Hill): C₂₈H₃₁IN₄O₅
• Peso molecular: 630.47
• Código UNSPSC: 12352202
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥98% (HPLC)
• forma: solution
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: dark red
• fluorescência: λ_ex ~560 nm; λ_em ~575 nm
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

Embalagem

Packaged under inert gas

Advertência

Toxicity: Irritant (B)

forma física

In DMSO.

Reconstituição

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

Outras notas

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Informações legais

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): 188.6 °F
• Ponto de fulgor (°C): 87 °C