251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• Sinônimo(s): DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• Fórmula empírica (Notação de Hill): C₂₅H₁₈F₂N₂O₇
• Número CAS: 254109-22-3
• Peso molecular: 496.42
• Número MDL: MFCD16872050
• Código UNSPSC: 12352204
• NACRES: NA.25

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥98% (HPLC)
• Formulário: solution
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; protect from light
• cor: pale yellow
• fluorescência: λ_ex ~500 nm; λ_em ~515 nm
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• chave InChI: BEVHTVRRVVEMEF-UHFFFAOYSA-N

Descrição

Descrição geral

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

Ações bioquímicas/fisiológicas

Primary Target
Nitric oxide (NO) fluorescent indicator

Advertência

Toxicity: Irritant (B)

forma física

A 5 mM solution in DMSO.

Reconstituição

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

Outras notas

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

Informações legais

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Ponto de fulgor (°C): 87 °C - closed cup - (Dimethylsulfoxide)