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Key Documents

CLS3464

Corning® Costar® 24 well plates

8.0 μm pore size, Tissue Culture (TC)-treated surface flat bottom clear polystyrene wells, round wells, clear plate, PET membrane, sterile, case of 48 ea, pack of 12 ea

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About This Item

Código UNSPSC:
41122107
NACRES:
NB.13

product name

Corning® Costar® Transwell® cell culture inserts, TC treated, PET membrane, diam. 6.5 mm, pore size 8.0 μm, sterile

Materiais

PET membrane
clear plate
flat bottom clear polystyrene wells
round wells

esterilidade

sterile

Características

lid
skirt

embalagem

pack of 12 ea
case of 48 ea

fabricante/nome comercial

Corning 3464

Diâmetro

6.5 mm

espessura da membrana × diâmetro

10 μm × 6.5 mm

tamanho

24 wells

volume de trabalho do poço

0.6 mL

cor

clear

tamanho de poro

8 μm
8.0 μm

tipo de ligação

Tissue Culture (TC)-treated surface

Descrição geral

The Transwell® cell culture inserts feature a distinctive self-centered and hanging design that effectively prevents the unwanted wicking of medium between the insert and outer well. This in turn ensures the uninterrupted and intact co-culturing of cells in the lower compartment, allowing for undisturbed cell growth and maintenance.

Aplicação

The Corning® Costar® Transwell® cell culture inserts are well-suited for cell staining applications and various chemotaxis assays, including fibroblast migration studies, tumor invasion and metastasis, trans-migrational studies, and invasion inhibition research. The inserts have been used in:
  • Transwell assay to study the role of TGF-β-MTA1-SMAD7-SMAD3-SOX4-EZH2 signaling axis in hepatocellular carcinoma cells
  • MTT Assay to study GAS5-mediated signaling in osteosarcoma cells
  • tri-cultured neuroinflammation model

Características e benefícios

  • 10 μm thick transparent polyester membrane
  • Treated for optimal cell attachment
  • Sterilized by gamma radiation Lids included TC-treated surface 0.33 cm2 cell growth area
  • Can be coated with a diverse range of extracellular matrices (ECM) and basement membrane extracts (BME)
  • Optical clarity enables clear visualization of cell outlines when observed under phase contrast microscopy
  • Offers compatibility with histochemical stains and fluorochromes for various cell staining applications

Informações legais

Corning is a registered trademark of Corning, Inc.
Costar is a registered trademark of Corning, Inc.
Transwell is a registered trademark of Corning, Inc.

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Growth Arrest Specific 5 (GAS5), a long noncoding RNA (lncRNA), functions as a tumor suppressor in multiple cancers. However, its function, downstream targets and upstream regulatory mechanism are still obscure in osteosarcoma cells. Here, we discovered that GAS5 was downregulated
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