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C7661

Sigma-Aldrich

Rat Collagen Type I

from rat tail, powder, suitable for cell culture

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About This Item

Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.75

product name

Colágeno, Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture

fonte biológica

rat tail

Nível de qualidade

linha de produto

BioReagent

forma

powder

peso molecular

120—160 kDa

embalagem

glass bottle of 5 mg

técnica(s)

cell culture | mammalian: suitable

cobertura de superfície

6‑10 μg/cm2

solubilidade

soluble

nº de adesão NCBI

nº de adesão UniProt

Especificidade de ligação

Peptide Source: Fibrinogen

Peptide Source: Laminin

temperatura de armazenamento

2-8°C

Informações sobre genes

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Descrição geral

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Col1a1 (collagen, type I, α1) or collagen is a major structural human protein, which assembles in the form of fibrils. It is a very long, thin and the most abundant protein found in the human body. It is a supercoiled right-helix of three left-handed polypeptide chains. These chains are composed of ~1040 amino acids, which are essentially repeats of three amino acids -(Gly-X-Y)n. Gly is glycine and X and Y can be any amino acids, but in humans are usually proline and hydroxyproline, respectively.
Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.

Aplicação

Collagen from rat tail is used for the following applications:

  • Immunohistochemistry
  • Cellular activity assays
  • Used in generation of dorsal root ganglion (DRG) explant cultures
  • Used as one of the components during the preparation of the functionalized surface (in NMR setup)
  • Used in cell culture (the glass coverslips were coated with nanowires at high concentrations mixed with collagen)
  • Used for biofunctionalization of the microchannels

Ações bioquímicas/fisiológicas

Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (collagenase type I) is linked with type I osteogenesis imperfecta. Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.Collagen from rat tail is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Nota de preparo

Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.

Outras notas

O colágeno é classificado em diversos tipos estrutural e geneticamente distintos. Usamos a nomenclatura proposta por Bornstein e Traub. Não confunda as designações de tipos da Sigma com tipos de classificação de colágeno reconhecidos.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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A major application of molecular MR imaging is receptor mapping of cells lining blood vessels with targeted contrast agents. Since these agents accumulate at interfaces, knowledge of their influence on the relaxation process in this specific configuration is a prerequisite
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