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Key Documents

125-50

SAFC

Collagen Coating Solution (50 ML)

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

Nível de qualidade

100

forma

liquid

prazo de validade

1 yr

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

Collagen is a major fibrous, structural protein in animals. It is a key component of the extracellular matrix (ECM) and is predominantly present in the connective tissue, ligaments, tendons, and cornea. Collagens consist of three parallel left-handed polypeptide chains coiled to form a right-handed triple helix. They also have a globular domain.

Aplicação

The coating concentration is 1 ml per 10 cm2 surface area of the cell culture ware: 2.5 ml for coating a T-25 flask or a 60 mm tissue culture dish. 7.5 ml for coating a T-75 flask or a 100 mm tissue culture dish.

Add Attachment Factor Solution to the flask and rock the flask gently to distribute the solution evenly to cover the whole culture surface. Coat the culture ware surface for 30 minutes at 37°C or 2 hours or overnight at room temperature. Remove Attachment Factor Solution by aspiration in a sterile hood. The coated flask can be used immediately or store at 4°C for up to 2 weeks.

Ações bioquímicas/fisiológicas

Collagen is a basic unit for the growth and support of various cells and tissues in the body. Collagen may be involved in wound healing. Collagen might promote cell adhesion and proliferation when used as a coating on iron-manganese alloys (Fe-30%Mn) scaffolds in orthopedic applications.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Protocolos

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Technical information for working with human follicle dermal papilla cells including thawing, subculturing and cryopreservation.

Human Skeletal Muscle Cells (HSkMC) Culture Protocol

Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation

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