C9301

Chicken Collagen Type II

from chicken sternal cartilage, powder, suitable for cell culture

• Número CAS: 9007-34-5
• Número CE: 232-697-4
• Número MDL: MFCD00130825
• Código UNSPSC: 12352202
• NACRES: NA.75

Propriedades

• Nome do produto: Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
• fonte biológica: chicken (Sternal cartilage)
• Nível de qualidade: 300
• tipo: Type II (Miller)
• linha de produto: BioReagent
• Formulário: powder
• embalagem: glass bottle of 100 mg; poly bottle of 25 mg; glass bottle of 5 mg
• concentração: 60-80% (biuret)
• técnica(s): cell culture | mammalian: suitable
• cobertura de superfície: 6‑10 μg/cm²
• solubilidade: acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
• nº de adesão UniProt: P02460
• Especificidade de ligação: Peptide Source: Fibrinogen
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: chicken ... COL2A1(395069)

Descrição

Descrição geral

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.

Aplicação

Collagen from chicken sternal cartilage has been used:

• in enzyme–linked immunosorbent assay (ELISA).
• chondrocyte-mediated tissue production in vitro.
• induction, treatment, and assessment of collagenα induced arthritis (CIA).
• cell proliferation assay.
• as a coating for cell culture surfaces.

Ações bioquímicas/fisiológicas

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

Características e benefícios

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

Outras notas

Major component of articular cartilage.

Nota de preparo

Prepared by a modification of the method of Trentham, D.E., et al.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)