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Documentos Principais

CC052

Sigma-Aldrich

Human Collagen Type II

liquid, 1 mg/mL, suitable for cell culture, used for gel formation

Sinônimo(s):

Collagen Type II, Human Collagen

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About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.75

Nome do produto

Human Collagen Type II,

fonte biológica

human

Nível de qualidade

Ensaio

90% (Human collagen type II)

Formulário

liquid

fabricante/nome comercial

Chemicon®

concentração

1 mg/mL

técnica(s)

cell culture | mammalian: suitable

Impurezas

<0.5% Non-collagen proteins
<1% Human collagen type IV
<1% Human collagen type V
<10% Human collagen type I
<10% Human collagen type III

entrada

sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)

solubilidade

water: soluble at 20 °C

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... COL2A1(1280)

Descrição geral

Type II collagen, a fibrillar collagen, is the main component of hyaline cartilage and constitutes 95% of the collagens and approximately 60% of cartilage’s dry weight. It is a homotrimer protein encoded by the gene COL2A1, mapped to human chromosome 12q13. Type two Collagen is also found in the nucleous pulposo of the intervertebral disc and the retina, sclera, and lens of the eye. Type IIA, is localized mainly to the vitreous body of the eye. Type IIB, is preferentially produced in adult cartilage tissue.  Collagen type II was purified by using differential salt precipitation, alcohol precipitation and DEAE chromatography. Multiple purification steps provide final preparations of collagen types in the native triple-helical form, free of any non-collagenous protein. Contamination of other collagen types in a standard preparation is lower than five percent.

Aplicação

Human Collagen Type II has been used:
  • as an extracellular matrix (ECM) protein in microarray analysis, to coat on nitrocellulose film slides for probing and detection experiments
  • to study the role of Col2a1a in the neural crest during early eye development in zebrafish model of Stickler syndrome
  • as a calibration standard in the quantification of collagen II levels in scaffolds using an enzyme-linked immunosorbent assay (ELISA)-based assay

Ações bioquímicas/fisiológicas

Type II collagen is commonly co-assembled with collagen XI, forming a complex that is covalently crosslinked to collagen IX and interacts with small leucine-rich proteoglycans. Collagen 2 imparts stability and strength to the tissue and maintains integrity and resilience under stress. Cleavage of Type II collagen occurs mainly through the action of matrix metalloproteinase collagenases, leading to the generation of well-known biomarkers such as the C-terminal telopeptide of Type II collagen and C2C. Mutations in type II collagen cause chondrodysplasia, leading to premature osteoarthritis.

forma física

Purified protein. Liquid in 0.5M acetic acid. No preservative.

Armazenamento e estabilidade

Aliquot and freeze. Maintain at -20°C up to 12 months. Avoid repeated freeze/thaw cycles.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Artigos

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Conteúdo relacionado

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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