C7774
Human Collagen Type I
from human placenta, powder, suitable for cell culture
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About This Item
Produtos recomendados
product name
Colágeno, Bornstein and Traub Type I (Sigma Type VIII), powder
fonte biológica
human placenta
Nível de qualidade
forma
powder
técnica(s)
cell culture | mammalian: suitable
Impurezas
HIV, hepatitis B and hepatitis C, none detected
temperatura de armazenamento
2-8°C
Informações sobre genes
bovine ... CSN2(281099) , CSN3(281728)
human ... COL4A3(1285) , COL4A4(1286) , COL4A6(1288)
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Aplicação
This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.
Ações bioquímicas/fisiológicas
Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.
Componentes
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.
Nota de preparo
Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.
Outras notas
O colágeno é classificado em diversos tipos estrutural e geneticamente distintos. Usamos a nomenclatura proposta por Bornstein e Traub. Tome cuidado para não confundir as designações de tipos da Sigma com tipos de classificação de colágenos reconhecidos.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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