C2888
Acetilcolinesterase
Type V-S, lyophilized powder, ≥1,000 units/mg protein
Sinônimo(s):
AChE, Acetilcolina acetil-hidrolase, Colinesterase, acetil
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About This Item
Produtos recomendados
fonte biológica
Electrophorus electricus
tipo
Type V-S
Formulário
lyophilized powder
atividade específica
≥1,000 units/mg protein
peso molecular
280 kDa
composição
Protein, ≥45% biuret
pI
~5.3
solubilidade
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
temperatura de armazenamento
−20°C
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Descrição geral
Peso molecular: 280 kDa
Ponto isoelétrico: 5,5
Coeficiente de extinção: E1% = 18,0 (280 nm)
A acetilcolinesterase de Electrophorus electricus é um tetrâmero composto por 4 subunidades iguais de 70 kDa cada. Cada subunidade contém um sítio ativo. A enzima é uma glicoproteína que contém hexosaminas.
Ponto isoelétrico: 5,5
Coeficiente de extinção: E1% = 18,0 (280 nm)
A acetilcolinesterase de Electrophorus electricus é um tetrâmero composto por 4 subunidades iguais de 70 kDa cada. Cada subunidade contém um sítio ativo. A enzima é uma glicoproteína que contém hexosaminas.
Aplicação
Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
- in acetylcholinesterase activity assay
- to study its effects on Xenopus embryonic hippocampal cells
- to study the effect of acetylcholine inhibitors on development and plasticity
Ações bioquímicas/fisiológicas
Enzima importante de degradação da acetilcolina in vivo. Converte acetilcolina + H2O em colina + ácido acético.
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Atenção
For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Definição da unidade
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
forma física
Lyophilized powder containing Tris buffer salts
Nota de análise
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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