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Documentos Principais

C2888

Sigma-Aldrich

Acetilcolinesterase

Type V-S, lyophilized powder, ≥1,000 units/mg protein

Sinônimo(s):

AChE, Acetilcolina acetil-hidrolase, Colinesterase, acetil

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About This Item

Número CAS:
Número da licença da enzima:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32

fonte biológica

Electrophorus electricus

Nível de qualidade

tipo

Type V-S

forma

lyophilized powder

atividade específica

≥1,000 units/mg protein

peso molecular

280 kDa

composição

Protein, ≥45% biuret

pI 

~5.3

solubilidade

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

temperatura de armazenamento

−20°C

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Descrição geral

Peso molecular: 280 kDa
Ponto isoelétrico: 5,5
Coeficiente de extinção: E1% = 18,0 (280 nm)

A acetilcolinesterase de Electrophorus electricus é um tetrâmero composto por 4 subunidades iguais de 70 kDa cada. Cada subunidade contém um sítio ativo. A enzima é uma glicoproteína que contém hexosaminas.

Aplicação

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
  • in acetylcholinesterase activity assay
  • to study its effects on Xenopus embryonic hippocampal cells
  • to study the effect of acetylcholine inhibitors on development and plasticity

Ações bioquímicas/fisiológicas

Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Enzima importante de degradação da acetilcolina in vivo. Converte acetilcolina + H2O em colina + ácido acético.

Atenção

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Definição da unidade

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

forma física

Lyophilized powder containing Tris buffer salts

Nota de análise

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Molecular and cellular neurosciences, 23(1), 96-106 (2003-06-12)
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