A4290
Anti-Human IgM (μ-chain specific) F(ab′)2 fragment−Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
Produtos recomendados
fonte biológica
goat
Nível de qualidade
conjugado
peroxidase conjugate
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
secondary antibodies
clone
polyclonal
Formulário
buffered aqueous solution
reatividade de espécies
human
técnica(s)
direct ELISA: 1:10,000
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Descrição geral
Goat polyclonal anti-Human IgM (μ-chain specific) F(ab′)2 fragment−Peroxidase antibody is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones Kappa and Bence Jones Lambda myeloma proteins.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu™ Red into detectable chromophores, light-emitters or fluorescers, respectively.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu™ Red into detectable chromophores, light-emitters or fluorescers, respectively.
Imunogênio
purified human IgM
Aplicação
Goat polyclonal anti-Human IgM (μ-chain specific) F(ab′)2 fragment−Peroxidase antibody may be used to detect and quantitate the level of IgM in human serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. ELISA assays on EBV +BCLs established from EBV+ human B cells were performed using HRP conjugated goat anti-human IgM mu chain specific as the secondary. Secondary antibody was incubated for 2 hrs at room temperature.
Peroxidise-conjugated goat F(ab′)2 fragment anti-human IgM (μ-chain specific) has been used as a secondary antibody to detect human IgMs to hantanvirus antigens using ELISA.
forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Nota de preparo
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Informações legais
Ampliflu is a trademark of Sigma-Aldrich Co. LLC
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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