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Documentos Principais

D5751

Sigma-Aldrich

Blue Dextran

Void volume marker for gel filtration columns.

Sinônimo(s):

Dextran blue from Leuconostoc mesenteroides

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About This Item

Número CAS:
Número MDL:
Código UNSPSC:
12352200
NACRES:
NA.25

fonte biológica

non-animal source

Formulário

powder

qualidade

200

peso molecular

2,000,000

técnica(s)

affinity chromatography: suitable

adequação

suitable for GFC marker

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Descrição geral

Blue Dextran is a soluble derivative of dextran. Its structure includes triazinyl dye Cibacron blue F3GA covalently bound to high molecular weight (2000 kDa) dextran. Blue Dextran is generally used as a marker to determine void volume in gel filtration chromatography columns.

Aplicação

Blue Dextran has been used for calibration of size exclusion chromatography (SEC) column. It has also been used in Dextran blue permeability assays to determine the integrity of co-cultures containing epithelial cells. Blue dextran may be used in affinity chromatography, gel filtration chromatography, protein chromatography, and molecular weight markers. Blue dextran is also suitable to study Alzheimer′s disease.

Nota de preparo

Prepared from dextran with an average mol. wt. of 2,000,000.

Nota de análise

degree of substitution: 0.10 to 0.12 mmol Cibracon Blue per gram of dextran

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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