A3547

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

• Sinônimo(s): ACP, Lysyl endopeptidase
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• fonte biológica: bacterial (LYSOBACTER SP. BACTERIA M497-1)
• Nível de qualidade: 200
• forma: lyophilized powder
• atividade específica: ≥1,000 units/mg solid
• peso molecular: 27 kDa
• técnica(s): cell based assay: suitable
• adequação: suitable for cell lysis
• aplicação(ões): diagnostic assay manufacturing
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Aplicação

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.

Ações bioquímicas/fisiológicas

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 °C.

Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀=0.6 over 2 hours at 37 °C.

Definição da unidade

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

forma física

crude powder

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P342 + P311
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Faceshields, Gloves