SAE0196
Achromopeptidase from bacteria
free of DNA contaminants, suitable for Microbiome research
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About This Item
Código UNSPSC:
12352204
Produtos recomendados
fonte biológica
bacterial
Nível de qualidade
forma
lyophilized powder
atividade específica
≥1000 U/mg
Características
DNA free
técnica(s)
DNA extraction: suitable
adequação
suitable for cell lysis
aplicação(ões)
cell analysis
Condições de expedição
ambient
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases. This product is free of detectable microbial DNA and is suitable for microbiome research
Aplicação
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Ações bioquímicas/fisiológicas
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. Optimum pH is 8.5-9.
Características e benefícios
Contains two bacteriolytic proteases for efficient lysis of Gram-positive bacteria and Gram-negative organisms
Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods
Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA
Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies
Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods
Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA
Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies
Definição da unidade
One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37 °C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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