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A2058

Sigma-Aldrich

Albumina sérica bovina

lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture

Sinônimo(s):

Albumina bovina, Albumina, soro bovino, BSA

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About This Item

Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.27

fonte biológica

bovine

linha de produto

BioReagent

Ensaio

≥95% (agarose gel electrophoresis)

forma

lyophilized powder

peso molecular

~66 kDa

purificado por

chromatography

embalagem

poly bottle of

origem

New Zealand origin

técnica(s)

cell culture | mammalian: suitable

Impurezas

IgG free
≤0.05% IgG
≤1 EU/mg endotoxin

pH

7

solubilidade

water: soluble (40 mg/ml)

nº de adesão UniProt

temperatura de armazenamento

2-8°C

Informações sobre genes

bovine ... ALB(280717)

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Descrição geral

Bovine serum albumin (BSA) is a non-glycosylated protein belonging to the serum albumins family. It is a globular protein with α-helix structure and 17-disulfide bonds. BSA has a molecular weight of 66 kDa consisting three domains and two subdomains under each. BSA shows structural similarity with human serum albumin.

Aplicação

BSA is widely used as a blocking agent in immunoassays and immunodetection procedures, and also as a carrier protein for dilution of antibodies. Thus it is of concern to be aware of potential trace endogenous content of globulins / IgG in BSA, because of concerns that such trace globulins / IgG may serve as antigens for secondary antibodies in immunodetection work. This product undergoes testing for low IgG content / status as essentially immunoglobulin-free.

Select the right albumin for your application with the Albumin Selection Guide
BSA is widely used as a blocking agent in immunoassays and immunodetection procedures, and also as a carrier protein for dilution of antibodies. Thus it is of concern to be aware of potential trace endogenous content of globulins / IgG in BSA, because of concerns that such trace globulins / IgG may serve as antigens for secondary antibodies in immunodetection work. This product undergoes testing for low IgG content / status as essentially immunoglobulin-free.

Select the right albumin for your application with the Albumin Selection Guide.
Bovine Serum Albumin has been used to treat polyomavirus BK to serve as a control in testing intravenous immunoglobulins preparations.

Ações bioquímicas/fisiológicas

Certos epítopos conformacionais e sequência primária de BSA são alérgenos suspeitos em alergias a carne de vaca e leite em humanos.
Bovine Serum Albumin (BSA) acts as a carrier protein for drugs, hormones and fatty acids. It is an essential component of cell culture medium. BSA serves as a blocking agent in enzyme linked immunosorbent assay (ELISA) and prevents non-specific binding of antigens and antibodies to the microtiter plates. Due to its high solubility, low cost and purity and interaction with surfactant, it is useful in pharmaceutical and cosmetic industry.

Características e benefícios

  • Tested for use in cell culture
  • Low endotoxin
  • Essentially IgG-free (immunoglobulin free)

Nota de preparo

Pode-se referir à albumina sérica como Fração V. Esta convenção de nomenclatura é derivada do método original de Cohn para fracionamento de proteínas séricas usando precipitação com etanol frio. A albumina sérica foi encontrada na quinta fração de etanol usando o método de Cohn. Desde então, o termo “Fração V” foi usado por alguns para descrever a albumina sérica, independentemente do método de preparo. Outros usaram este termo para descrever a albumina sérica purificada por métodos de fracionamento com etanol que foram altamente modificados desde o método de Cohn original foi descrito. A Sigma-Aldrich fabrica e distribui albuminas séricas purificadas a partir de diversos métodos primários, inclusive o método de fracionamento de Cohn verdadeiro, métodos de fracionamento de etanol modificados, choque térmico e cromatografia. Etapas de purificação adicionais podem incluir cristalização ou filtração de carvão.
Prepared by salt fractionation, ion exchange, and gel filtration chromatography.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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Artigos

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

rganoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

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