ROAMYGLL

Amyloglucosidase

from Aspergillus niger

• Sinônimo(s): Aspergillus niger, disaccharidase-type a-glucosidase
• Número da licença da enzima: 3.2.1.3 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: Aspergillus niger
• Nível de qualidade: 100
• forma: lyophilized
• atividade específica: 6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate); ~6 units/mg protein (At 25 °C with glycogen as the substrate.)
• peso molecular: 97 kDa
• embalagem: pkg of 3,500 U (11202367001); pkg of 500 U (11202332001)
• fabricante/nome comercial: Roche
• pH ideal: 4.6-4.8
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Especificidade

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Aplicação

Amyloglucosidase from Aspergillus niger is used for the determination of starch.

Ações bioquímicas/fisiológicas

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Nota de preparo

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:

• Western blot: 1 to 10 μg/ml

Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash