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Key Documents

A1602

Sigma-Aldrich

Amiloglicosidase

ammonium sulfate suspension, ≥40 units/mg protein

Sinônimo(s):

1,4-α-D-Glicano glico-hidrolase, Exo-1,4-α-glicosidase, Glicoamilase

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About This Item

Número CAS:
Número da licença da enzima:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

forma

ammonium sulfate suspension

Nível de qualidade

atividade específica

≥40 units/mg protein

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

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Descrição geral

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Aplicação

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.

Ações bioquímicas/fisiológicas

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Definição da unidade

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

forma física

Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Artigos

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolos

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

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