AP180P
Donkey Anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed
Chemicon®, from donkey
Sinônimo(s):
Anti-Rabbit IgG HRP, HRP Donkey Anti-Rabbit IgG
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Produtos recomendados
fonte biológica
donkey
Nível de qualidade
conjugado
peroxidase conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
secondary antibodies
clone
polyclonal
reatividade de espécies
goat
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Descrição geral
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Aplicação
Donkey anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in ELISA, IH & WB.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
Qualidade
Routinely tested on Enzyme-linked Immunosorbent Assay.
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.
forma física
Purified donkey secondary antibody IgG in lyophilized buffer 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitution:
Reconstitute with 500 μL of sterile distilled water.
Reconstitution:
Reconstitute with 500 μL of sterile distilled water.
Armazenamento e estabilidade
Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
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